Background: CCR5-delta32 heterozygous individuals are susceptible to HIV-1. However, it is not clear if there is a\r\nrelevant protective effect against transmission and a beneficial effect in terms of HIV progression which cannot be\r\nattributed to CCR5 surface density alone. Therefore we investigated HIV-1 dependency factors (HDF) which might\r\nbe differently regulated in CCR5 wild type (WT) and CCR5-delta32 heterozygous individuals.\r\nMethods: We examined CD34+ hematopoietic progenitor cells derived from bone marrow samples from 19\r\nhealthy volunteers, 12 individuals with CCR5 WT and 7 with heterozygous CCR5-delta32 deletion. Samples were\r\nanalyzed using a global gene expression oligonucleotide microarray (HG-U133plus 2.0, Affymetrix Inc.).\r\nResults: A total of 205 genes were found with altered expression (3fold difference, present call rate of 75%,\r\np < 0.05) and 7 of these had a connection to HIV-1 pathogenesis. In 4 genes: TOP1, CXCR2, SREBF2, and TAP we\r\nfound a different regulation which was consistent with a supposed beneficial effect for CCR5-delta32 heterozygotes.\r\nConclusion: The CCR5-delta32 deletion is associated with other HDFs in HIV-1 pathogenesis as a possible\r\nexplanation for beneficial effects regarding the deletion leading to a variant expression profile in heterozygous\r\ncarriers of this mutation.
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